Trying to split a txt file into multiple variables

So I'm making a program where it reads a text file and I need to separate all the info into their own variables. It looks like this:

>1EK9:A.41,52; B.61,74; C.247,257; D.279,289
ENLMQVYQQARLSNPELRKSAADRDAAFEKINEARSPLLPQLGLGAD
YTYSNGYRDANGINSNATSASLQLTQSIFDMSKWRALTLQEKAAGIQ
DVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLDQTT
QRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGN
YYPELAALNVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLARE
QIRQAQDGHLPTLDLTASTGISDTSYSGSKTRGAAGTQYDDSNMGQN
KVGLSFSLPIYQGGMVNSQVKQAQYNFVGASEQLESAHRSVVQTVRS
SFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIVDVLDAT
TTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPV
STNPENVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN

The code after the > is a title, the next bit that looks like this "A.41,52" are numbered positions in the sequence I need to save to use, and everything after that is an amino acid sequence. I know how to deal with the amino acid sequence, I just need to know how to separate the important numbers in the first line.

In the past when I just had a title and sequence I did something like this:

for line in nucfile:
if line.startswith(">"):
    headerline=line.strip("n")[1:]
else:
    nucseq+=line.strip("n")

Am I on the right track here? This is my first time, any advice would be fantastic and thanks for reading :)